Figure 2.

Flowchart of FusionQ. FusionQ constructs the reference transcriptome directly from the RNA-Seq data, and detects the two types of fusion reads based on it.‚ÄČThe proposed residual sequence extension method makes the short residual sequence longer, and therefore has a higher probability for unique mapping and increases the specificity of the detection results. The chimerical transcript quantification is based on the RSEM algorithm.

Liu et al. BMC Bioinformatics 2013 14:193   doi:10.1186/1471-2105-14-193
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