The paper prototyping method was used for usability testing of initial designs for the Enzyme Portal. The user was presented with a paper wireframe of each screen interface and could interact with it using a pencil instead of a mouse and keyboard. Each screen would then change according to the interaction. The wireframe interface designs were created using Balsamiq software (http://balsamiq.com webcite).
de Matos et al. BMC Bioinformatics 2013 14:103 doi:10.1186/1471-2105-14-103