This article is part of the supplement: Eleventh International Conference on Bioinformatics (InCoB2012): Bioinformatics
The study of muscle remodeling in Drosophila metamorphosis using in vivo microscopy and bioimage informatics
1 Live-Cell Imaging and Automation of Image Analysis Group, Imaging Informatics Division, Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR), Singapore
2 Department of Biological Sciences, National University of Singapore (NUS), Singapore
BMC Bioinformatics 2012, 13(Suppl 17):S14 doi:10.1186/1471-2105-13-S17-S14Published: 13 December 2012
Temporal comparisons of time-lapse images facilitate the discovery of phenotypic abnormalities resulting from genetic perturbations. The movie shows metamorphosis of two genotypes at 30 minute intervals, a control specimen (left panel) expressing Grasp65-GFP (green) and Histone-mKO (red) and specimen expressing EAST(1-1902)-GFP (right panel), tau-GFP (green) and Histone-mKO (red). The time-lapse starts during the prepupal stage (-6 hours) and last until late pupal stage (+88 hours). The transition from the prepupal to pupal stage begins at zero hours. Note that EAST(1-1902)-GFP overexpression lead to suppression of muscle histolysis in the first 25 hours of the pupal stage and abnormalities of muscle morphology from +38 hours onwards. See Figure 3 for more details.
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