Analysis work flow for isotope dilution MRM-MS data with and without the use of AuDIT. After LC-MRM-MS analysis of samples, transition peaks are identified and integrated with software from either the mass spectrometer vendor or another supplier. (A), Flow of data with use of the automated algorithm. The statistical test identifies problem transitions from the variation in the relative ratios for the analyte and the SIS. The CV of the PARs is used as a filter to flag transitions with unacceptably large variation. (B), The current standard practice of careful manual inspection of all transitions by an expert. Adapted from Abbatiello, Mani, et. al., Clinical Chemistry, 56, 291-305 .
Mani et al. BMC Bioinformatics 2012 13(Suppl 16):S9 doi:10.1186/1471-2105-13-S16-S9