Study of breast cancer cell lines. Two cultures from two breast cancer cell lines (low-invasive MCF7 and high-invasive Hs578T) were treated with two oxygen levels (normoxia, hypoxia) for two periods of time (6 and 24 hours). We refer to each combination of these treatments as condition. Separate cultures were grown in each condition, and therefore the experiment had a 3-way factorial experimental design.
Clough et al. BMC Bioinformatics 2012 13(Suppl 16):S6 doi:10.1186/1471-2105-13-S16-S6