Comparison of quality score assignment. Comparison of quality score assignment. (A) Observed versus predicted quality score. Both HPCall and the native 454 base-caller have an equally accurate quality score assignment, whereas Pyrobayes performs clearly worse. (B) Cumulative proportion of called bases versus the assigned quality score. HPCall assigns more high quality scores than the other base-callers; the left figure suggests that this assignment is done accurately.
Beuf et al. BMC Bioinformatics 2012 13:303 doi:10.1186/1471-2105-13-303