Figure 3.

Coverage of the designed primers over a Tm range. The dataset of 247 FASTA sequences were input into MCMC-ODPR with no SNP processing and 10,000 optimization iterations. The percentage sequence coverage of target amplicons is plotted over a melting temperature range.

Kitchen et al. BMC Bioinformatics 2012 13:287   doi:10.1186/1471-2105-13-287
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