Coverage of the designed primers over a Tm range. The dataset of 247 FASTA sequences were input into MCMC-ODPR with no SNP processing and 10,000 optimization iterations. The percentage sequence coverage of target amplicons is plotted over a melting temperature range.
Kitchen et al. BMC Bioinformatics 2012 13:287 doi:10.1186/1471-2105-13-287