Additional file 2.

Figure S2. Flowchart showing steps in data simulation and model assessment. In step 2, the differences in data generation are caused by two factors: (i) imprinted genes need to express only one allele at a tissue level while non-imprinted genes don’t, (ii) two alleles expressed from non-imprinted genes need to be sequenced in RNA-Seq with an equal probability, while imprinted genes only have one allele expressed. In this step we also need to assume that sequencing error leads to misread of one nucleotide to the other three with an equal probability. RT-PCR amplification is not shown in the process because we assume that it amplifies both alleles synchronously (for details, see Discussion)

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Li et al. BMC Bioinformatics 2012 13:271   doi:10.1186/1471-2105-13-271