Consistency of red blood cell lysis across staining panels. If red blood cells are not properly lysed, they will be detected as events in the FCM experiment. Under ideal conditions, only white blood cells would be detected. The outlier threshold is set such that at least 80% of events should be within the white blood cell gate. Between one and two samples within each staining panel were identified as having lower than expected red blood cell lysis efficiency. Closer inspection revealed these to be from the same coresampleid.
Finak et al. BMC Bioinformatics 2012 13:252 doi:10.1186/1471-2105-13-252