QA result of of MFI stability vs time for the FITC channel. We can see clear examples where the MFI is not stable over time, i.e. it is either increasing (HLADR, CD8, CD11c), or decreasing (LD). Some stains show residuals that are not normally distributed, suggesting non-linear trends (HLADR, CD8, CD57), while others are generally stable with the occasional sample outlier (CD1c, IgG1, 6B11). The formula used to generate the plot is: MFI∼RecdDt|channel∗stain, where the MFI is plotted against RecdDt, the date the sample was run, which is defined in the study metadata. Channel and stain are generated upon parsing the workspace. Outlier calls are done within each combination of channel and stain. An additional argument to plot(..,subset=channel='FITC',..) tells the function to plot only the output for the FITC channel. The population for this qaTask is the population, MFI, which selects all MFI gates.
Finak et al. BMC Bioinformatics 2012 13:252 doi:10.1186/1471-2105-13-252