A graphical representation of ΔGcut-off and of ΔG vs. Probe Signal. Top panel shows ΔGcut-off results: the probes having ΔG < −5.2 kcal mol-1 fall to the left of the red line, these were filtered out (21.5 %). Bottom panel shows ΔG vs. Probe signal: the red line denotes background cut-off value; the grey line is the Lowess smoothing line between ΔG and log10 intensities; grey dots represent the probes with very stable structures that have been eliminated in the process; black dots represent the probes with signal higher than the background cut-off value and ΔG < −5.2 kcal mol-1.
Baciu et al. BMC Bioinformatics 2012 13:244 doi:10.1186/1471-2105-13-244