Table 1

Minimal spine population (number of cells per group) which guarantees false negative rate below 5%
Modeled change t-test p-value Parameter studied Required number of cells per group
in spines variable (with 15, 30, 60 spines/cell)
10% 0.001 area 166 84 43
length 137 70 35
head-width 66 35 19
20% 0.001 area 47 24 14
length 39 21 13
head-width 21 12 8
50% 0.001 area 13 8 6
length 11 7 5
head-width 7 5 4
10% 0.01 area 119 62 31
length 101 51 27
head-width 49 26 14
20% 0.01 area 33 19 10
length 29 15 8
head-width 15 9 6
50% 0.01 area 9 6 4
length 8 5 4
head-width 5 4 3

Simulation results with various numbers of cells, spines per cell, p-values of the test, strength of systematic change and parameters studied were used to set the limits on number of analyzed cells per group in order to guarantee that the false negative rate falls below 5%. The systematic changes were modeled by the linear growth. The test groups were compared by Student’s two-tailed t-test.

Ruszczycki et al.

Ruszczycki et al. BMC Bioinformatics 2012 13:213   doi:10.1186/1471-2105-13-213

Open Data