Additional file 1.

PrimerPairsFromQuantPrime.doc Primer pairs generated from QuantPrime. Fifty-two randomly selected human template sequences from NCBI Refseq mRNA database are used to generate target-specific primers by QuantPrime. The “SYBR Green real-time qPCR (no splice variant hits)” option was selected with the organism set to human and the database set to “RefSeq 04/30/09 (reference assembly)(genome+) (splice variants)”. Default values were used for all other options. The underlined pairs are used as example cases in Figure 5.

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Ye et al. BMC Bioinformatics 2012 13:134   doi:10.1186/1471-2105-13-134