Figure 5.

Examples of potential unintended targets for primer pairs generated by QuantPrime and PRIMEGENS. Example targets are extracted from Primer-BLAST specificity checking results for primer pairs generated by QuantPrime or PRIMEGENS (a total of 162 and 116 potential unintended targets were identified for QuantPrime and PRIMEGENS, respectively. See Table 2 for details). The example primers correspond to those underlined in Additional files 1 and 2.