Example results of designing target-specific primers. Note that while five primer pairs were returned (as shown in graphic summary), due to space limitation, the figure shows details for the first primer pair only. The “Search Summary” link, when clicked, shows the search parameters used as well as the total number of BLAST hits that are generated during the search process (355,744 hits for the current search). Numbers in alignments indicate the start and end positions for primer and target. A dot (.) indicates nucleotide identity to primer sequence. The search was done on 11/02/2011.
Ye et al. BMC Bioinformatics 2012 13:134 doi:10.1186/1471-2105-13-134