Nearest-neighbor computation example. The duplex is composed of 3 patterns: two perfectly matching patterns (patterns 1 and 3) and one single mismatch (pattern 2). To compute the enthalpy and entropy of the perfectly matching patterns, MELTING 5 uses a single thermodynamic formula based on user-defined or default thermodynamic parameters. Other thermodynamic formula and parameters are used to compute the enthalpy and entropy of pattern 2.
Dumousseau et al. BMC Bioinformatics 2012 13:101 doi:10.1186/1471-2105-13-101