Email updates

Keep up to date with the latest news and content from BMC Bioinformatics and BioMed Central.

Open Access Methodology article

Robust joint analysis allowing for model uncertainty in two-stage genetic association studies

Dongdong Pan12, Qizhai Li2*, Ningning Jiang2, Aiyi Liu3 and Kai Yu4

Author Affiliations

1 Department of Statistics, Yunnan University, Kunming 650091, PR China

2 Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100190, PR China

3 Biostatistics and Bioinformatics Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA

4 Biostatistics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD 20892, USA

For all author emails, please log on.

BMC Bioinformatics 2011, 12:9  doi:10.1186/1471-2105-12-9

Published: 7 January 2011

Abstract

Background

The cost efficient two-stage design is often used in genome-wide association studies (GWASs) in searching for genetic loci underlying the susceptibility for complex diseases. Replication-based analysis, which considers data from each stage separately, often suffers from loss of efficiency. Joint test that combines data from both stages has been proposed and widely used to improve efficiency. However, existing joint analyses are based on test statistics derived under an assumed genetic model, and thus might not have robust performance when the assumed genetic model is not appropriate.

Results

In this paper, we propose joint analyses based on two robust tests, MERT and MAX3, for GWASs under a two-stage design. We developed computationally efficient procedures and formulas for significant level evaluation and power calculation. The performances of the proposed approaches are investigated through the extensive simulation studies and a real example. Numerical results show that the joint analysis based on the MAX3 test statistic has the best overall performance.

Conclusions

MAX3 joint analysis is the most robust procedure among the considered joint analyses, and we recommend using it in a two-stage genome-wide association study.