Additional file 7.

Validation data set results comparison between MFP and Sanger sequencing. (A) Call rate improvement when comparing MFP analysis to GSEQ 4.1 for each of 5 validation samples. (B) Accuracy between total calls made by MFP analysis and Sanger sequencing for each of 5 validation samples. (C) Call type details for a total of 5 discrepant calls between MFP analysis and Sanger sequencing seen in 3 of the validation samples. Interestingly, 3 of the 5 discrepant calls involved the same SNP (A12307G) in three distinct samples (C5, C9, C11) that was detected by MFP. (D) Sanger-based electropherogram clearly shows the presence of the A > G variation, not at 12307, but at its neighboring position 12308, as shown here for validation sample C5.

Format: PPT Size: 176KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Xie et al. BMC Bioinformatics 2011 12:402   doi:10.1186/1471-2105-12-402