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This article is part of the supplement: UT-ORNL-KBRIN Bioinformatics Summit 2010

Open Access Poster presentation

The UofL Microarray Facility: merger and new location

S Waigel1*, Xiaohong Li3, Yinlu Chen3, Vennila Arumugam1, Nigel GF Cooper3 and Wolfgang Zacharias12

Author Affiliations

1 J. G. Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA

2 Dept. of Medicine and Dept. of Pharmacology & Toxicology, University of Louisville, Louisville, KY 40202, USA

3 Department of Anatomical Sciences & Neurobiology, University of Louisville School of Medicine, Louisville, KY 40202, USA

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BMC Bioinformatics 2010, 11(Suppl 4):P25  doi:10.1186/1471-2105-11-S4-P25

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2105/11/S4/P25


Published:23 July 2010

© 2010 Waigel et al; licensee BioMed Central Ltd.

Background

The UofL Microarray Facility is centered around the currently most advanced and comprehensive instrumentation for oligonucleotide array platforms by Affymetrix, Inc. and Agilent, Inc., both merged in the new location in CTR 227H-G. These technologies allow determination of global RNA gene expression and SNP (single nucleotide polymorphism) profiles based on hybridization of entire representative mRNA or genomic DNA populations to high-density arrays of gene-specific or mutation-specific oligonucleotide probes. Such global profiles can be obtained for tens of thousands of genes or hundreds of thousands of SNP loci from cell or tissue samples within one time-saving and standardized experiment. Comparative analysis of global gene expression or SNP patterns on biological material will provide crucial information on gene products and/or mutations which are involved in cellular functions, disease mechanisms, and therapy responses. Both cell culture, tissue, or microdissected cell material from human or various animal sources can be used for analyses. The objective of the UofL Microarray Facility is to ensure that investigators can efficiently adopt these state-of-the-art technologies for their basic science, clinical, and translational investigative goals. The Facility is registered as Academic Core Account and receives discounts for arrays and software licenses.

Basic services:

RNA expression profiling

Single-nucleotide polymorphism genotyping

Splice variation analyses

microRNA profiling

Quantitative and qualitative nucleic acid analysis

DNA sequencing

The Facility also provides DNA sequencing services and offers assistance with quantitative and qualitative nucleic acid analysis using the Agilent BioAnalyzer Lab-on-Chip Technology and the Nanodrop ND-1000 low-volume spectrophotometer. The Facility also provides free access to the most current statistics and data analysis software versions of Partek Genomics Suite, Ingenuity, and Genespring.

NEW LOCATION

The merged Microarray Facility is now located in Rooms 227F-H of the new CTR Building, 505 South Hancock Street.

Website

http://louisville.edu/research/genomics/ webcite

Contacts

Please visit our website for details and pricing, or contact us:

Facility Personnel

Sabine Waigel, Facility Manager: 852-3798; sjwaig01@louisville.edu

Xiaohong Li, Senior Research Technologist, Sequencing Facility Manager: 852-4690; x0li0013@louisville.edu

Vennila Arumugam; Research Technologist II

Yinlu Chen; Research Analyst Sr./Biostatistics

Co-Directors

Dr. Wolfgang Zacharias: 852-2579; w0zach01@lousville.edu

Dr. Nigel Cooper: 852-1474; ngcoop01@gwise.louisville.edu

Acknowledgements

The UofL Microarray Facility is supported by NIH/COBRE Molecular Targets P20RR018733, NIH/KBRIN P20 RR16481, the J. G. Brown Foundation, and user fees.