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Resolution: standard / high Figure 1.
Distribution of read start position as a percentage length for genes with median length
of 1200 nucleotides. The red line is a cubic spline fit. (A) Illumina dataset - RNA fragmentation by sonication
[10] (dataset 1), (B) Roche 454 dataset - cDNA fragmentation by nebulization [14], and (C) Simulated cDNA fragmentation assuming fragments in the size range of 500-800
nucleotides.
Howard and Heber BMC Bioinformatics 2010 11(Suppl 3):S6 doi:10.1186/1471-2105-11-S3-S6 |