Comparison of biological, library preparation, and flow-cell effects. Boxplots of estimated log-fold-changes for UHR vs. Brain biological effects (GLM 2 in [Additional file 2: Supplemental Table S4]), flow-cell effects adjusting for biology (GLM 4), library preparation effects within flow-cell (GLM 7). Estimates are presented for total-count (black) and upper-quartile (blue) normalization.
Bullard et al. BMC Bioinformatics 2010 11:94 doi:10.1186/1471-2105-11-94