Abstract
Background
Recent technological advancements have made high throughput sequencing an increasingly popular approach for transcriptome analysis. Advantages of sequencingbased transcriptional profiling over microarrays have been reported, including lower technical variability. However, advances in technology do not remove biological variation between replicates and this variation is often neglected in many analyses.
Results
We propose an empirical Bayes method, titled Analysis of Sequence Counts (ASC), to detect differential expression based on sequencing technology. ASC borrows information across sequences to establish prior distribution of sample variation, so that biological variation can be accounted for even when replicates are not available. Compared to current approaches that simply tests for equality of proportions in two samples, ASC is less biased towards highly expressed sequences and can identify more genes with a greater log fold change at lower overall abundance.
Conclusions
ASC unifies the biological and statistical significance of differential expression by estimating the posterior mean of log fold change and estimating false discovery rates based on the posterior mean. The implementation in R is available at http://www.stat.brown.edu/Zwu/research.aspx webcite.
Background
Recent technological advancements have made high throughput sequencing an increasingly popular approach for transcriptome analysis. Unlike microarrays, enumeration of transcript abundance with sequencing technology is based on direct counts of transcripts rather than relying on hybridization to probes. This has reduced the noise caused by crosshybridization and the bias caused by the variation in probe binding efficiency. Sequencingbased transcription profiling does have other challenges. For example, whole transcript analysis produces data with transcript length bias [1]. Other biases, including GC content, have also been reported [2]. Nonetheless, sequencing based expression analysis has been shown to be more robust and have higher resolution compared to microarray platforms [3]. Some researchers have predicted that it will eventually replace microarrays as the major platform for monitoring gene expression [4]. The importance of replicates is well recognized in microarray analysis [5] and it is now standard practice to include biological replicates under each experimental condition. However, as of now, sequencingbased gene expression studies often do not include replicates [68], posing the question of whether the biological variation is, or can be, adequately addressed.
For illustration, we use data from Illumina Digital Gene Expression (DGE) tag profiling in this paper. However, our statistical methodology, and its implementation in R, are general for all sequencingbased technologies that quantify gene expression as counts instead of continuous measurements such as probe intensity in microarrays. In DGE, the 3' end of transcripts with a polyA tail are captured by beads coated with oligo dT. Two restriction enzymes, NlaIII and Mmel are used to digest the captured transcripts, generating a 21base fragment starting at the most 3' NlaIII site. The 21base fragments are sequenced to quantify the transcriptome. Consider two samples in a comparison and let X_{1 }and X_{2 }be the counts of a particular sequence tag in the two samples. The most common approach is to consider the counts as a realization of binomial distribution B(N_{i}, π_{i}), i = 1,2. where N_{i }is the total number of sequences in a sample, representing sequencing depth. A statistical test for π_{1 }= π_{2 }can be conducted. The classical Ztest using the Gaussian approximation to the binomial distribution is proposed for the Serial Analysis of Gene Expression (SAGE) data [9,10] and recently applied to DGE and other sequencing data [1113], and Fisher's exact test has also been proposed [14]. In other technologies, sequence counts may have to be combined at either the exon or full transcript level to form the counts X_{1 }and X_{2}.
The test for H_{0 }: π_{1 }= π_{2 }can be performed without replicates. However, rejection of the H_{0 }hypothesis simply implies difference between the two samples. Unless the proportion of a gene in the transcriptome is the same for all samples under the same condition (lack of withinclass variation), we can not generalize the difference between two samples to the difference between two classes. The withinclass biological variation among replicates leads to over dispersion in Binomial or Poisson models. Models accounting for over dispersion, such as a betabinomial, have been introduced for the analysis of SAGE data when several replicates within each class are available [15,16]. Robinson and Smyth [17] use a negative binomial model and squeeze tagwise dispersion towards a common dispersion, estimated using all tags, with a weighted likelihood approach that yields an EBlike solution. edgeR [18], a Bioconductor package implementing this method, has been applied to both DGE and RNAseq data with replicates. However, since replicates are still rare in high throughput sequencing, many researchers have been relying on simple tests of equal proportion with multiple testing correction. Another drawback in some current analysis methods, especially those applied to the noreplicate situation, is the use of Gaussian approximation of binomial distribution [11,19], which does not work well with data that include low count numbers. In transcriptome analysis, due to the depth of sequencing, the majority of genes have low counts. Relying on Gaussian distribution often gives highly expressed genes favorable statistical power, such that genes that have a lower expression but exhibit greater extent of differential expression between samples are less likely to be discovered.
In this paper we present an empirical Bayes method, titled Analysis of Sequence Counts (ASC), to estimate the log fold change of transcription between two samples. We borrow information across sequences to estimate the hyper parameters representing the normal biological variation among replicates and the distribution of a transcriptome. The statistical model does not rely on Gaussian approximation of the binomial distribution for all tags and requires no special treatment of 0 counts. Differential expression is computed in the form of a shrinkage estimate of log fold change. This estimate is the basis for ranking genes. We also compute the posterior probability that the log fold change is greater than a biologically relevant threshold chosen by the user. In contrast to sorting genes simply by pvalues, we focus on the biological significance (represented by the posterior expectation of log fold change) and provide uncertainty measure in the form of posterior probability.
Modeling biological variation
It has been reported that the noise in gene expression by sequencing depends on expression level as observed in microarray data [19]. It has been widely observed that the scatter plot of the log readspermillion (rpm) between samples have wider spread for lower average counts, as shown in Figure 1A. This is shown by the relationship between the empirical variance of log rpm across replicates and the average of log rpm. For example, Stolovitzky et al [19] binned genes whose average rpm are closest and computed sample standard deviation (SD) of genes within each bin, and reported higher SD in bins with smaller rpm. However, the sample SD is only expected to be a good estimate of the biological variation of log expression when the Gaussian approximation works well. We conducted a simulation in which the biological variation of log expected rpm is constant, and the observed rpm is generated from a Poisson distribution. The sample SD of log rpm, as shown in Figure 1B, also appears to be inflated for low expression genes. This demonstrates that the inflated variance in observed log rpm does not necessarily imply higher biological variation, but often is a result of poor approximation of a Binomial random variable by a Gaussian distribution. Figure 2 shows that the quantilequantile plots of the differences of observed log rpm (i.e., the observed log fold change) in T. pseudonana data (see Methods) at various average expression levels. The straight lines in all plots have the same slope, suggesting stable biological variation. The fact that most of the points stay on the straight line also confirms that a Gaussian approximation is a reasonable choice for the biological variation.
Figure 1. Scatter plot of the log_{10 }rpm in two samples. A. Scatter plot of the log_{10 }rpm in two samples. The greater spread at lower counts suggests higher variance. B. Sample variance of log_{10 }rpm from a simulation with constant biological variation shows that the increased sample variance may be caused by poor approximation of a Gaussian distribution to a Binomial distribution for sparse counts. To create the simulation dataset, we sampled log(π_{0}) from observed average log counts and created log(π_{1}) = log(π_{0}) + δ/2 and log(π_{2}) = log(π_{0})  δ/2 where δ ~ N (0, σ^{2}). In this plot σ = 0.122, as estimated in the example data.
Figure 2. Quantile quantile (QQ) plots of the differences of log rpm (log(p_{1})  log(p_{2})) confirming the Gaussian distribution as a reasonable approximation of the biological variation. A.QQ plots of log(p_{1})  log(p_{2}) for genes with average log rpm within 2 ± 0.05. B) For genes with average log rpm within 1.6 ± 0.05 C) For genes with average log rpm within 1.2 ± 0.05 D) For genes with average log rpm within 0.8 ± 0.05. The straight lines in all figures have a slope 0.28, suggesting that biological variation does not depend on average rpm.
Distribution of expression levels in a transcriptome
As observed in both microarray data and sequencingbased transcriptome profiling, genes can differ by orders of magnitude in their expression levels, ranging from less than 1 per million to thousands per million and the majority of genes have relatively low counts. Tags with 0 counts cause problems in statistical analyses that take a direct log transformation and some investigators have had to develop special treatments for those genes [19]. In Figure 3 we show the empirical distribution of the average log rpm, defined as [log_{10}(x_{1 }∧ 0.5)/N_{1 }+ log_{10}(x_{2 }∧ 0.5)/N_{2}] /2. This is a highly skewed distribution even in the log scale. The skewness motivates us to use a shifted exponential distribution as the prior distribution for the average expression level.
Figure 3. Histogram of the average log_{10 }rpm between the A and B samples. Histogram of the average log_{10 }rpm between the A and B samples. The smooth curve shows the probability density function of the fitted shifted exponential distribution.
Results and Discussion
We applied ASC to transcription profiles of the diatom Thalassiosira pseudonana under two culturing conditions, measured by DGE, and computed the posterior expectation of log fold change for all genes. Figure 4 compares the shrinkage estimate with the "apparent log fold change" based on sample proportions. In order to display all tags including those with count 0 in log scale, we define the apparent log ratio as . We also define the average proportion for each gene as the geometric mean of the two proportions, with similar adjustment at 0. This adjustment at 0 is for the completeness of visual presentation, and is not done in ASC analysis. The estimated fold change from genes with high counts are almost the same as the apparent fold change, but genes with sparse counts are shrunk more aggressively (Figure 4). This is a desirable property since the coefficient of variation of binomial distribution decreases with the expectation. This implies that when the expected count is small, it is much easier to produce counts with apparent large fold changes.
Figure 4. Shrinkage estimate of log fold change. Shrinkage estimate of log fold change from ASC plotted against apparent log fold change for all genes. The apparent log fold change is defined as where x* = x ∧ 0.5 to avoid logarithm of 0 counts. The darkness of each point reflects the average rpm, , of a gene, with the darkest being the highest average rpm. The straight line is the identity line representing no shrinkage. The genes with lighter gray have lower average rpm and are shrunk more.
We estimated the posterior mean of log fold change and the posterior probability that there is greater than two fold change for a given tag. There are 1050 genes with posterior probability greater than 0.9 that the fold change is greater than 2. The average log rpm of those tags spread from less than 0.23 (1.7 rpm) to 3.6 (10,000 rpm) and most have approximately 1 (10 rpm). Figure 5A highlights these genes in a scatter plot of observed log rpm. F5B shows the distribution of average rpm of these genes, suggesting that majority of genes displaying differential expression by ASC analysis have moderate counts, even though we have aggressively shrunk the estimate of log fold change for tags with low counts.
Figure 5. Differential expressed genes identified by ASC. A. Scatter plot of the log_{10 }rpm in two samples. Differentially expressed genes with posterior probability of fold change greater than 2 are highlighted in red. B. Distribution of average rpm for the highlighted differentially expressed genes.
Comparison with other methods
All of the genes identified as differentially expressed by ASC have very small pvalues if a simple test of equal proportions is performed. In fact, a simple Ztest identifies 3479 differentially genes at significance level 0.05 with Bonforonni correction, as highlighted in red in Figure 6A. Fisher's exact test gives almost the same results except adding a few more genes with less total tag counts. We highlight the top 1000 genes with smallest pvalues in blue, and it is clear that genes with lower average expression are not identified. Figure 6B shows that the majority of genes identified to have differential expression have much higher average counts compared to those identified by ASC. We have also applied a software, DGEseq [11], recently developed specifically for the analysis of digital gene expression data as in this example. DGEseq identified more than 7000 genes with estimated FDR less than 0.01, and also favors transcripts with higher average rpm. The results are included in additional file 1, Figure S1.
Figure 6. Differential expressed genes identified by Ztest. A. Scatter plot of the log_{10 }rpm in the A and B samples. Differentially expressed genes with Bonferonni adjusted pvalue less than 0.05 highlighted in red and the smallest 1000 of which highlighted in blue. B. Distribution of average rpm for the highlighted red or blue genes.
Additional file 1. Figure S1. A. Scatter plot of the log_{10 }rpm in the A and B samples. Differentially expressed genes identified by DGEseq with estimated qvalue (Storey FDR) less than 0.01 highlighted in red and the genes with smallest qvalue 1000 of which highlighted in blue. B. Distribution of average rpm for the highlighted red or blue genes.
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ASC clearly prioritizes genes differently from Z test or DGEseq and finds more genes with modest expression but greater fold change as differentially expressed. In order to show that the top ranked genes in ASC are associated with higher biological significance, we obtained DGE data from an experiment comparing expression from two genotypes with 4 replicates each [3] (GSE10782). Hoen et al [3] compute Bayes Error using SAGE BetaBin [16], a method that takes into account of biological variation between replicates. The Bayes Error in SAGE BetaBin represents the "superposition" between the estimated posterior distributions of the classes in comparison and is used to rank the genes for differential expression. To evaluate how various methods work when replicates are not available, we choose the first sample of each genotype and obtained lists of the top 1000 tags and compared the Bayes Error for those tags based on the full data. Among the top 1000 tags found by ASC, 320 have an estimated Bayes Error of approximately 0, significantly more than those found by the other statistics (Table 1).
Table 1. Overlap between the top 1000 genes identified by different methods and the SAGE BetaBin ranking.
We have also used edgeR [18], a moderated statistical test for sequencing data with replicates [17,20], to analyze the full dataset. For the no replicate case, we first used the two samples as replicates to estimate the dispersion parameter in edgeR and then estimated differential expression given that dispersion. We compared the overlap between the top ranked differentially expressed genes using edgeR on the full data (using edgeR) and top ranked genes from the data without replicates (using ASC, edgeR, DGEseq, Z and fisher's exact test). The agreement between ASC and full data edgeR is almost identical to the agreement between noreplicate and full data edgeR, while the latter is expected since it is based on the same methodology. About a third of the top 100 genes identified in the full data are recovered in the top 100 genes ranked by ASC. In contrast, less than 2 of the top 100 genes from full data analysis made to the top 100 list by the other methods. The comparison is summarized in Table 2.
Table 2. Overlap between the top 100 or top 1000 differentially expressed genes identified by edgeR on full data and by other statistics on data without replicate
Why is there so little overlap between the top genes by Ztest on two sample comparison and the top genes from edgeR analysis on the full data set? Strikingly, many genes with extreme pvalues in a Ztest have small fold changes. This is because there is greater statistical power to detect even subtle changes in gene expression when the counts are higher. From the Gaussian approximation to the sample proportion , we have for large N_{π}, the log sample proportion is also approximately Gaussian, . Since the expected counts N_{π }varies greatly from a few to over a hundred thousand, and the variance of log sample proportion decreases sharply with the increase of expected counts, it is clear that statistical power is biased towards genes with higher counts. This also causes the bias of higher power towards longer transcripts in full transcript analysis. An extreme pvalue in such a test only suggests that the proportions of a transcript is significantly different between the two samples of comparison, not whether the difference is beyond what is reasonable between biological replicates. Figure 7 overlays the distribution of apparent log fold changes for the top 1000 genes identified by either simple Ztest or ASC. Since ASC identifies a gene as differentially expressed only when a shrinkage estimate of log fold change is above certain level, the apparent log fold change from sample proportions are always away from 0. On the other hand, a simple Ztest can identify many genes with very small changes. It appears that DGEseq fails to adequately account for biological variation and the results from DGEseq are very similar to that from a Ztest (Additional file 2, Figure S2).
Figure 7. Histogram of the apparent fold change of the top 1000 genes found by Ztest or ASC.
Additional file 2. Figure S2. Histogram of the apparent fold change of the top 1000 genes found by DGEseq or ASC.
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Discussion
We present a simple hierarchical model for sequencingbased gene expression data (e.g. DGE, RNAseq ect.) that provides a shrinkage estimate of differential expression in the form of posterior mean of log fold change. Even in experiments lacking replicates, we take advantage of the large number of sequences quantified in the same experiment and establish a prior distribution of difference between conditions. The differential expression of a gene is evaluated based on the posterior expectation of log fold change. This estimate takes into account the increased uncertainty for genes with smaller counts (demonstrated by more aggressive shrinking in Figure 4) yet still allows the identification of differential expression among genes with lower expression. Our measure of statistical uncertainty is the posterior probability that the differential expression is beyond a given threshold, thus the inference on differential expression avoids the problem of conflicting "statistical significance" versus "biological significance" seen in Ztests.
It is not uncommon to use hierarchical models for gene expression data. Several models used in microarray data analysis [21,22] add another level of hierarchy by assuming that only a fraction of genes may have been affected by any treatment, and the rest have absolutely no change. Therefore, δZ = 1 ~ N(0, τ^{2}) and δ = 0Z = 0 with P(Z = 1) = p_{0}. This essentially assumes that the prior distribution of δ is a zeroinflated Gaussian distribution. We show that using a simple Gaussian prior provides good shrinkage without the extra layer of hierarchy, which greatly simplifies computation.
In biological terms, our model means that the mean gene expression levels between two populations are never absolutely equal for any gene. However, the difference for most genes are small. We use posterior expectation as the estimate of the magnitude of difference. McCarthy and Smyth [23] showed that testing the differential expression relative to a biologically meaningful threshold identifies more biologically meaningful genes. We take a similar approach and estimate the posterior probability that the differential expression is greater than a threshold. Therefore, we avoid genes with very subtle differential expression even if that difference is statistically significant between the two samples in comparison. The genes identified by ASC include those that are modestly expressed as well as highly expressed.
Methods
DGE data generation
The diatom Thalassiosira pseudonana (Strain 1335 from the Center for the Culture of Marine Phytoplankton) was grown axenically in 24 hr light at 14°C in f/2 media [24,25] made with Sargasso Sea water, Treatments consisting of phosphoruslimited medium (0.4 μM PO_{4}) and phosphorusreplete medium (36 μM PO_{4}) were grown in triplicate and are herein referred to as treatments A and B, respectively. Equal volumes of cell biomass from each replicate were pooled for the A or B treatments 96 hours after inoculation and harvested by gentle filtration. Filters were immediately frozen in liquid nitrogen and stored at 80°C.
Total RNA was extracted using the RNeasy Midi Kit (Qiagen), following the manufacturer's instructions with the following changes: RNA samples were processed with Qiashredder columns (Qiagen) to remove large cellular material and DNA was removed with an oncolumn DNAase digestion using RNasefree DNAase (Qiagen). A second DNA removal step was conducted using the Turbo DNAfree kit (Ambion, Austin, TX, USA)[B1]. The RNA was quantified in triplicate using the Mx3005 Quantitative PCR System (Stratagene) and the QuantiT RiboGreen RNA Assay Kit (Invitrogen) and was analyzed for integrity by gel electrophoresis. Total RNA was sent to Illumina (Hayward, CA) and they constructed digital gene expression (DGE) libraries with NlaIII tags following their protocol. Sequencing libraries for NlaIII digested tags were constructed by Illumina and sequenced on their Genome Analyzer. 12,525,833 tags were sequenced from the A library and 13,431,745 tags were sequenced from the B library.
Hierarchical model for gene counts
For each transcript, we assume the observed sequence counts follow a Binomial distribution given its expected expression under a biological condition. For a sequencing run that yields total count N for all sequence fragments, the expected count for gene i is expressed as Nπ where π is the expected proportion of this gene in the transcriptome. For two samples in the comparison, we observe the counts x_{1 }and x_{2 }while
Many researchers simply test π_{1 }= π_{2 }and perform a Bonferonni correction to account for multiple testing. We reparametrize π_{1 }and π_{2 }as follows:
Here δ has the interpretation of log fold change in gene expression, and λ is a nuisance parameter representing the average (log) expression.
We assume prior distributions
where Exp represents shifted exponential distribution with rate α and shift λ_{0}.
The posterior distribution of the differential expression is therefore
We obtain the posterior mean given the gene counts as an estimate of differential expression. We refer to as the shrinkage estimate of log fold change, which is sufficient to rank the genes. To evaluate the statistical significance, we compute the posterior probability P(δ > Δ_{0}x), where Δ_{0 }is a userdefined effect size of biological significance. There is no closed form expression for the posterior distribution and we use numerical integration for the evaluation of the posterior mean and probability.
Estimation of hyper parameters
The observed log rpm has a very skewed distribution, motivating us to use a distribution with exponential decay. But the location of this distribution is shifted compared to exponential distribution with an unknown lower bound. One advantage of the exponential distribution is the closed form expression of its cumulative density function. For λ ~ Exp(α, λ_{0}), . From the sequence counts, we first compute average log rpm between the two conditions and use these to obtain the empirical CDF . Thus for two quantiles q_{1}, q_{2}, we can obtain empirical quantiles and and . Solving the equations
We can also use the method of moments to estimate the rate without knowing the shift parameter since the conditional expectation also has a closed from, due to the lack of memory property. For a given 0 < q < 1,
Thus we can estimate α as . Our default setting is q_{1 }= 0.8 and q_{2 }= 0.9. The posterior mean E[δx] is not sensitive to the choice of q_{1}, q_{2 }(Additional file 3, Figure S3). Again, due to the lack of memory property, the probability density of shifted exponential distributions with the same rate are proportional, thus the value of λ_{0 }does not affect the posterior distribution and does not need to be estimated.
Additional file 3. Figure S3. Sensitivity of to hyperparameter estimation. Scatter plot of and , based on q_{1 }= 0.8, q_{2 }= 0.9 and q_{1 }= .9, q_{2 }= 0.95, respectively. The maximum difference in estimated fold change is less than 0.04, indicating that is not sensitive to the choice of q.
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To estimate τ, the parameter representing the biological variation among replicates, we borrow information across genes. Although for any given gene we only observe one total count under each condition, and thus the true differential expression and the biological variation cannot be identified, we assume that the majority of genes are not affected by the treatment, an assumption found to be reasonable in microarray data in many experiments. We can model τ as a function of λ, but Figure 2 suggests that the biological variation is rather constant across expression levels. Thus we estimate one global parameter τ. We start with the Gaussian approximation of the Binomial model . Since the total counts N are usually a very large integer, we also have, approximately, . The variance of log(p_{1}) decreases with rate of 1/N as π increases and becomes negligible compared to biological variation. Thus we simply estimate τ from the differences of log rpm with the highest average log rpm. We use inter quartile range instead of sample standard deviation to avoid influence of genes with extreme differential expression. . In practice we use total counts above 1000 and this allows us to have several thousand genes (over 4000 in our example) for the estimation.
Authors' contributions
ZW developed the statistical methodology, in consultation with BDJ and TAR, and drafted the manuscript. BDJ, TAR, STD and MAS designed the study that generated the DGE data, and contributed to writing the manuscript. MM and LPW performed experiments that generated the DGE data. All authors have read and approved the final manuscript.
Acknowledgements
We thank the reviewers for their insightful comments and suggestions that greatly strengthened the manuscript. We thank A. Drzewianowski for her assistance with laboratory experiments. Funding was provided by NSF OCE0723677.
References

Oshlack A, Wakefield M: Transcript length bias in RNAseq data confounds systems biology.
Biology Direct 2009, 4:14. PubMed Abstract  BioMed Central Full Text  PubMed Central Full Text

Dohm J, Lottaz C, Borodina T, Himmelbauer H: Substantial biases in ultrashort read data sets from highthroughput DNA sequencing.
Nucleic acids research 2008, 36(16):e105. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Hoen P, Ariyurek Y, Thygesen H, Vreugdenhil E, Vossen R, de Menezes R, Boer J, van Ommen G, den Dunnen J: Deep sequencingbased expression analysis shows major advances in robustness, resolution and interlab portability over five microarray platforms.
Nucleic acids research 2008, 36(21):e141. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Li B, Ruotti V, Stewart R, Thomson J, Dewey C: RNASeq gene expression estimation with read mapping uncertainty.
Bioinformatics 2010, 26(4):493. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Lee M, Kuo F, Whitmore G, Sklar J: Importance of replication in microarray gene expression studies: statistical methods and evidence from repetitive cDNA hybridizations.
Proceedings of the National Academy of Sciences of the United States of America 2000, 97(18):9834. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Cheng L, Lu W, Kulkarni B, Pejovic T, Yan X, Chiang J, Hood L, Odunsi K, Lin B: Analysis of chemotherapy response programs in ovarian cancers by the nextgeneration sequencing technologies.
Gynecologic Oncology 2010, 117:159169. PubMed Abstract  Publisher Full Text

Marti E, Pantano L, BanezCoronel M, Llorens F, MinonesMoyano E, Porta S, Sumoy L, Ferrer I, Estivill X: A myriad of miRNA variants in control and Huntington's disease brain regions detected by massively parallel sequencing.

Cui L, Guo X, Qi Y, Qi X, Ge Y, Shi Z, Wu T, Shan J, Shan Y, Zhu Z, Wang H: Identification of microRNAs Involved in the Host Response to Enterovirus 71 Infection by a Deep Sequencing Approach.
Journal of Biomedicine and Biotechnology 2010, 2010:425939. Publisher Full Text

Kal A, Van Zonneveld A, Benes V, Van Den Berg M, Koerkamp M, Albermann K, Strack N, Ruijter J, Richter A, Dujon B, et al.: Dynamics of gene expression revealed by comparison of serial analysis of gene expression transcript profiles from yeast grown on two different carbon sources.
Molecular biology of the cell 1999, 10(6):1859. PubMed Abstract  PubMed Central Full Text

Schaaf G, van Ruissen F, van Kampen A, Kool M, Ruijter J: Statistical comparison of two or more SAGE libraries.
Methods in Molecular Biology 2008, 387:151168. PubMed Abstract  Publisher Full Text

Wang L, Feng Z, Wang X, Wang X, Zhang X: DEGseq: an R package for identifying differentially expressed genes from RNAseq data.
Bioinformatics 2010, 26:136. PubMed Abstract  Publisher Full Text

Nygaard S, Jacobsen A, Lindow M, Eriksen J, Balslev E, Flyger H, Tolstrup N, Møller S, Krogh A, Litman T: Identification and analysis of miRNAs in human breast cancer and teratoma samples using deep sequencing.
BMC Medical Genomics 2009, 2:35. PubMed Abstract  PubMed Central Full Text

Hashimoto S, Qu W, Ahsan B, Ogoshi K, Sasaki A, Nakatani Y, Lee Y, Ogawa M, Ametani A, Suzuki Y, et al.: HighResolution Analysis of the 5'End Transcriptome Using a Next Generation DNA Sequencer.
PLoS One 2009, 4:e4108. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Bloom J, Khan Z, Kruglyak L, Singh M, Caudy A: Measuring differential gene expression by short read sequencing: quantitative comparison to 2channel gene expression microarrays.
BMC genomics 2009, 10:221. PubMed Abstract  BioMed Central Full Text  PubMed Central Full Text

Baggerly K, Deng L, Morris J, Aldaz C: Differential expression in SAGE: accounting for normal betweenlibrary variation.
Bioinformatics 2003, 19(12):1477. PubMed Abstract  Publisher Full Text

Vêncio R, Brentani H, Patrão D, Pereira C: Bayesian model accounting for withinclass biological variability in Serial Analysis of Gene Expression(SAGE).
BMC bioinformatics 2004, 5:119. PubMed Abstract  BioMed Central Full Text  PubMed Central Full Text

Robinson M, Smyth G: Moderated statistical tests for assessing differences in tag abundance.
Bioinformatics 2007, 23(21):2881. PubMed Abstract  Publisher Full Text

Robinson M, McCarthy D, Smyth G: edgeR: a Bioconductor package for differential expression analysis of digital gene expression data.
Bioinformatics 2010, 26:139. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Stolovitzky G, Kundaje A, Held G, Duggar K, Haudenschild C, Zhou D, Vasicek T, Smith K, Aderem A, Roach J: Statistical analysis of MPSS measurements: Application to the study of LPSactivated macrophage gene expression.
Proceedings of the National Academy of Sciences of the United States of America 2005, 102(5):1402. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Robinson M, Smyth G: Smallsample estimation of negative binomial dispersion, with applications to SAGE data.
Biostatistics 2008, 9(2):321. PubMed Abstract  Publisher Full Text

Smyth GK: Linear models and empirical Bayes methods for assessing differential expression in microarray experiments.
Statistical Applications in Genetics and Molecular Biology 2004, 3:Article 3. Publisher Full Text

McCarthy D, Smyth G: Testing significance relative to a foldchange threshold is a TREAT.
Bioinformatics 2009, 25(6):765. PubMed Abstract  Publisher Full Text  PubMed Central Full Text

Guillard R, Ryther J: Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran.
Canadian Journal of Microbiology 1962, 8:229. PubMed Abstract  Publisher Full Text

Guillard R: Culture of phytoplankton for feeding marine invertebrates.