The enolase superfamily (ES) motif. (a) The substructure responsible for the conserved partial reaction among members of the ES consists of the five residues shown above from which the ES motif used here is derived . (b) Enolase from Saccharomyces cerevisiae [PDB:1ELS] with demonstrated divalent ion coordination to the conserved superfamily substructure . A single Mn2+ is coordinated by a carboxylate triad (Asp, Glu, Asp in this enzyme) and a second Mn2+ binds in a substrate specific manner .
Moll et al. BMC Bioinformatics 2010 11:555 doi:10.1186/1471-2105-11-555