Illustration of bivariate manual gating by a flow cytometry expert: Dr John Zaunders of the Centre for Immunology, Sydney, Australia. The gates correspond to the red-coloured shapes. The flow cytometry data correspond to a study on graft-versus-host disease (source: Brinkman et al. ). The panels correspond to day number with respect to blood and marrow transplant of a particular patient. The vertical axis is sinh-1(side-scatter), whilst the horizontal axis is sinh-1(fluorescence) for the second channel. Since the data are large flowViz defaults to displaying the data as smoothed scatterplots, based on bivariate kernel density estimation.
Naumann et al. BMC Bioinformatics 2010 11:44 doi:10.1186/1471-2105-11-44