RT-PCR analysis of the novel ncRNA candidates in group I. (A) ncRNAs predicted to be transcribed in only one direction. Reverse transcription was performed using Oligo dT primers. 1: SEQ1; 2: SEQ2; 3: SEQ3; 4: SEQ4; 5: SEQ13; 6: SEQ5a or SEQ5b; 7: SEQ6a or SEQ6b; 8: SEQ7a or SEQ7b; 9: SEQ8a or SEQ8b; 10: SEQ5a combined with SEQ6a or SEQ5b combined with SEQ6b; 11: SEQ14; 12: SEQ15; 13: SEQ16; 14: SEQ17; 15: SEQ18a or SEQ18b. (B) ncRNAs predicted to be transcribed in both directions. Reverse transcription was performed using primers complement to the predicted ncRNAs. Here, -RT denotes negative control.
Song et al. BMC Bioinformatics 2009 10(Suppl 1):S36 doi:10.1186/1471-2105-10-S1-S36