Characterisation and correction of signal fluctuations in successive acquisitions of microarray images

doi:10.1186/1471-2105-10-98

BMC Bioinformatics

Volume 10

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Glatigny A
Delacroix H
Tang T
François N
Aggerbeck L
Mucchielli-Giorgi MH

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Tang T
François N
Aggerbeck L
Mucchielli-Giorgi MH
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Methodology article

Characterisation and correction of signal fluctuations in successive acquisitions of microarray images

Annie Glatigny¹ , Hervé Delacroix¹^,2 , Thomas Tang¹^,3 , Nicolas François¹^,2^,4 , Lawrence Aggerbeck¹ and Marie-Hélène Mucchielli-Giorgi¹^,3

¹Centre de Génétique Moléculaire, CNRS FRE3144, F-91198 Gif-sur-Yvette, France

²Université Paris-Sud 11, F-91405 Orsay, France

³Université Pierre et Marie Curie – Paris 6, F-75005 Paris, France

⁴TELECOM & Management SudParis, 9 rue Charles Fourier, F-91011 Evry, France

author email corresponding author email

BMC Bioinformatics 2009, 10:98doi:10.1186/1471-2105-10-98


Published:	30 March 2009

Additional files

Additional file 1:

Fluctuation, (e), of the M values of "outlier" spots within a series of scans for slide #3. This figure represents the fluctuation (e) of the M values of "outlier" spots within a series of scans for the red channel and the green channel (part A and part B, respectively) and for images obtained with one, two or three acquisitions per line (lines with circles, squares and triangles, respectively).

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Additional file 2:

The standard deviation of each pixel of "non-outlier" and of "outlier" spots. This figure shows the standard deviation of each pixel of the spots obtained from 8 successive scans with one, two or three acquisitions per line (from left to right). The height of each vertical bar corresponds to the standard deviation of one pixel within the scan series, graduations are indicated by white broken lines. Part A3, A4 and A5 are for "non outlier" spots and B3, B4 and B5 for "outlier" spots (F532_adj).

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