Resolution:
## Figure 3.
Pairwise Comparisons of Different Normalized Data Sets. Pairwise comparisons between the three different normalization methods and the non-normalized
dataset. The graphs represent the log variance ratios for each gene versus its average
Ct value. The red line is the smoothed lowess curve that captures the overall trend
of the data in the plot. The dotted blue line represents horizontal axis. The direction
of the ratio is reflected in each individual figure title, e.g. the ratios in Figure
3.3A are constructed by taking the log_{2 }transformation of the GAPDH-normalized variance divided by the non-normalized variance
for each gene. Points below the dotted blue line correspond to those genes where single
gene GAPDH normalization has resulted in a greater reduction in variance relative
to the variance of these genes in the non-normalized data.
Mar |