|
Optimal production of the twelve biosynthetic precursors and the metabolic cofactors. |
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| Compound |
μmol/g DW |
Compound |
μmol/g DW |
|
|
|||
| Amino Acids |
Phospholipids |
||
| Alanine |
488 |
Phosphatidyl serine |
2.58 |
| Arginine |
281 |
Phosphatidyl ethanolamine |
96.75 |
| Asparagine |
229 |
Phosphatidyl glycerol |
23.22 |
| Aspartate |
229 |
Cardiolypin |
6.45 |
| Cysteine |
87 |
Fatty acid composition (% of total fatty acid) |
|
| Glutamate |
250 |
Myristic acid (2.68) |
|
| Glutamine |
250 |
Myristoleic acid (7.70) |
|
| Glycine |
582 |
Palmitic acid (38.23) |
|
| Histidine |
90 |
Palmitoleic acid (10.74) |
|
| Isoleucine |
276 |
Heptadecenoic acid (16.11) |
|
| Leucine |
428 |
cis-Vaccenic acid (0.90) |
|
| Lysine |
326 |
Oleic acid (17.91) |
|
| Methionine |
146 |
Nonadecenoic acid (5.73) |
|
| Phenylalanine |
176 |
Cell Wall Sructures |
|
| Proline |
210 |
Lipopolysaccharide |
8.4 |
| Serine |
205 |
Peptidoglycan |
27 |
| Threonine |
241 |
Cofactors and other molecules |
|
| Tryptophan |
54 |
5-Methyl-THF |
50.0 |
| Tyrosine |
131 |
Putrescine |
35.0 |
| Valine |
402 |
Spermidine |
7.0 |
| Protein synthesis/processing (ATP/ Amino Acid) |
4.306 |
NAD |
2.15 |
| Ribonucleotides |
NADH |
0.05 |
|
| ATP |
165 |
NADP |
0.13 |
| GTP |
203 |
NADPH |
0.4 |
| CTP |
126 |
UDP-Glucose |
3.0 |
| UTP |
136 |
ATP |
4.0 |
| RNA synthesis/processing (ATP/Nucleotide) |
0.40 |
ADP |
2.0 |
| Deoxyribonucleotides |
AMP |
1.0 |
|
| dATP |
24.7 |
CoA |
0.03 |
| dTTP |
24.7 |
Acetyl-CoA |
0.04 |
| dGTP |
25.4 |
Succinyl-CoA |
0.01 |
| dCTP |
25.4 |
Glycogen |
154 |
| DNA synthesis/processing (ATP/Nucleotide) |
1.372 |
||
|
The optimal production was calculated for the wild-type strain and the deletion strains. The constraints are set as defined in Figure 1. The color code quantitatively defines effect of the in silico deletion; red corresponds to 0.0, yellow corresponds to 0-50% of the wild-type production, blue corresponds to 50-100% of the wild-type, and no color coding is illustrated when the production is unchanged from the wild-type. Data for other carbon sources is available online. G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; R5P, ribose-5-phosphate; E4P,erythrose 4-phosphate; T3P1, glyceraldehyde 3-phosphate; 3PG, 3-phosphoglycerate; PEP, phosphoenolpyruvate; PYR, pyruvate; ACCOA, acetyl-CoA; AKG, α-ketoglutarate; SUCCOA, succinyl-CoA; OA, oxaloacetate. | |||
Edwards and Palsson BMC Bioinformatics 2000 1:1 doi:10.1186/1471-2105-1-1 |
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