Soluble protein is incorporated into fibers more efficiently in chimeras with increased number of repeats in the ORD. Fiber formation reactions (5 μM) were separated into supernatant (S) and pellet (P) fractions by high speed centrifugation and analyzed by SDS-PAGE and western blot using α-Sup35 antibodies. Soluble protein remains in the supernatant fraction and the fibers are found in the pellet fraction. (T) is the total reaction prior to centrifugation.
Kalastavadi and True BMC Biochemistry 2008 9:7 doi:10.1186/1471-2091-9-7