Sup35NM and SP5NM are efficiently cross-seeded by SP14NM seeds. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Reactions were seeded with 2.5% (w/w) of preformed sonicated fibers as indicated. Graphs are plotted as %RFU versus time and are representative of three experiments. (A) Fiber formation of Sup35NM. (B) Fiber formation of SP5NM.
Kalastavadi and True BMC Biochemistry 2008 9:7 doi:10.1186/1471-2091-9-7