|
Biochemical and kinetic properties of inulosucrase (IslA) from L. citreum CW28 and truncated versions. |
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| Truncated version |
Optimum T (°C) |
Half life @ 35°C (min) |
Hydrolysis/transglycosylase ratio (%)a |
KmT (mM) |
kcat (s-1) |
kcat/Km (mM-1 s-1) |
|
|
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| IslA |
35 |
420 |
40/60 |
38 |
25 |
0.65 |
| IslA2 |
35 |
407 |
38/62 |
24 |
28.19 |
1.17 |
| IslA3 |
30 |
346 |
61/39 |
nd |
nd |
nd |
| IslA4 |
30 |
128 |
70/30 |
142 |
105 |
0.73 |
|
a Measured after depletion of 293 mM sucrose. KmT (total Km) was determined from sucrose consumption rate. IslA3 did not follow a Michaelis Menten kinetic behavior | ||||||
del Moral et al. BMC Biochemistry 2008 9:6 doi:10.1186/1471-2091-9-6 |
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