Figure 4.

Mutation of Rad23 F9 reduces interaction with Ufd2. GST-pulldown experiments were performed by mixing each purified Rad23 protein with GST-Ufd2 bound to glutathione-sepharose beads. After mixing at 4°C for 1 hour, the resin was washed, and bound proteins were eluted and analyzed by SDS-PAGE and Western blotting for Rad23.