An earthworm protease cleaving serum fibronectin and decreasing HBeAg in HepG2.2.15 cells

doi:10.1186/1471-2091-9-30

BMC Biochemistry

Volume 9

Viewing options:

Abstract
Full text
PDF (469KB)
Additional files

Associated material:

Related literature:

Articles citing this article
on Google Scholar
on PubMed Central
Other articles by authors
on Google Scholar

Wang XQ
Chen L
Pan R
Zhao J
Liu Y
He RQ

on PubMed

Wang XQ
Chen L
Pan R
Zhao J
Liu Y
He RQ
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Post to:

Research article

An earthworm protease cleaving serum fibronectin and decreasing HBeAg in HepG2.2.15 cells

Xue-Qing Wang¹^,2^,3 , Lan Chen¹ , Rong Pan¹ , Jing Zhao¹ , Ying Liu¹ and Rong-Qiao He¹^,3

¹State Key Lab of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, 15 Da Tun Road, Chao Yang District, Beijing 100101, PR China

²School of Pharmaceutical Sciences, Peking University, 38 Xue Yuan Road, Hai Dian District, Beijing 100191, PR China

³Graduate University of Chinese Academy of Science, 19 A Yuquan Road, Shijingshan District, Beijing 100049, PR China

author email corresponding author email

BMC Biochemistry 2008, 9:30doi:10.1186/1471-2091-9-30


Published:	24 November 2008

Additional files

Additional file 1:

Digestion of human serum in the presence of EFNase. EFNase (final concentrations as indicated) was incubated with human serum (25 μl) at 37°C for 30 min, and then aliquots were taken for SDS-PAGE (panel A). Serum in the present of trypsin was used as positive control (panel B).

Format: PDF Size: 52KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 2:

The identification of 'fibronectin' in the serum using mass spectrometry. The peptide fingerprint of 'fibronectin' was analyzed by MALDI-TOF MS after digested by trypsin.

Format: PDF Size: 361KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 3:

The mass/charge and sequences of matched peptides in the determination of 'fibronectin' in the serum using mass spectrometry.

Format: DOC Size: 45KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 4:

Digestion of fibronectin by EFNase. EFNase (final concentrations as indicated) was incubated with FN at 37°C for 15 min, and then aliquots were taken for reducing SDS-PAGE (panel A). BSA in the presence of EFNase was used as a control (panel B).

Format: PDF Size: 41KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 5:

Immunoblotting of EFNase in the medium of serosal side during mucosal-to-serosal transport and immunohistochemistry analysis of the intestinal epithelium. The everted sac model for studying intestinal transport of large peptides and proteins has been used here. Mucosal-to-serosal transport (duodenum segment of small intestine) of EFNase was performed. Aliquots (5 μL) of serosal medium were taken at different incubation time intervals for immunoblotting as indicated (panel A). Lane 6 indicated the full-sized EFNase as a positive control. The gray shade densities of immunoreactive bands were shown on panel B. Incubated with EFNase (final concentration 10 μM) at the mucosal side for 30 min, the everted intestinal segment was sectioned and immunologically visualized in the presence of anti-EFNase serum as primary antibody (panel C). Those in the absence of the primary antibody were used as controls (panel D). The results indicated that the intact EFNase could be transported from the mucosal to serosal side.

Format: PDF Size: 56KB Download file

This file can be viewed with: Adobe Acrobat Reader