Immunoaffinity purification and characterization of mitochondrial membrane-bound D-3-hydroxybutyrate dehydrogenase from Jaculus orientalis

doi:10.1186/1471-2091-9-26

BMC Biochemistry

Volume 9

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Mountassif D
Andreoletti P
El Kebbaj Z
Moutaouakkil A
Cherkaoui-Malki M
Latruffe N
El Kebbaj MS

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Mountassif D
Andreoletti P
El Kebbaj Z
Moutaouakkil A
Cherkaoui-Malki M
Latruffe N
El Kebbaj MS
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Methodology article

Immunoaffinity purification and characterization of mitochondrial membrane-bound D-3-hydroxybutyrate dehydrogenase from Jaculus orientalis

Driss Mountassif¹^,2 , Pierre Andreoletti¹ , Zakaria El Kebbaj² , Adnane Moutaouakkil³^,4 , Mustapha Cherkaoui-Malki¹ , Norbert Latruffe¹ and M'hammed Saïd El Kebbaj²

¹INSERM U866 (Institut National de la Santé et de la Recherche Médicale), Université de Bourgogne, LBMC (Biochimie Métabolique et Nutritionnelle), Faculté des Sciences, 6 Bd Gabriel, 21000 Dijon cedex, France

²Laboratoire de Biochimie et Biologie Moléculaire, Université Hassan II – Aïn Chock, Faculté des Sciences Aïn Chock, km 8 route d'El Jadida BP. 5366, Mâarif, Casablanca, Morocco

³Laboratoire de Physiologie et Génétique Moléculaire, Université Hassan II – Aïn Chock, Faculté des Sciences Aïn Chock, km 8 route d'El Jadida BP. 5366, Mâarif, Casablanca, Morocco

⁴Unité de Radio-Immuno-Analyse, Département des Applications aux Sciences du Vivant, CNESTEN (Centre National de l'Energie, des Sciences et des Techniques Nucléaires), BP 1382 RP, 10001 Rabat, Morocco

author email corresponding author email

BMC Biochemistry 2008, 9:26doi:10.1186/1471-2091-9-26


Published:	30 September 2008

Additional files

Additional file 1:

Determination of BDH molecular weight in denaturing conditions of electrophoresis

Format: EPS Size: 994KB Download file

Additional file 2:

Influence of temperature in the activation-denaturation processes of purified BDH from Jerboa