Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapods

doi:10.1186/1471-2091-9-24

BMC Biochemistry

Volume 9

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Haskins N
Panglao M
Qu Q
Majumdar H
Cabrera-Luque J
Morizono H
Tuchman M
Caldovic L

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Haskins N
Panglao M
Qu Q
Majumdar H
Cabrera-Luque J
Morizono H
Tuchman M
Caldovic L
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Research article

Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapods

Nantaporn Haskins¹ , Maria Panglao¹^,2 , Qiuhao Qu¹^,3 , Himani Majumdar¹ , Juan Cabrera-Luque¹ , Hiroki Morizono¹ , Mendel Tuchman¹ and Ljubica Caldovic¹

¹Research Center for Genetic Medicine, Children's National Medical Center, 111 Michigan Ave NW, Washington DC, 20010, USA

²School of Medicine and Health Sciences, The George Washington University, 2300 I St. NW, Washington DC, 20037, USA

³Division of Neurosciences, Beckman Research Institute, 1450 E Duarte Road, Duarte, CA 91010, USA

author email corresponding author email

BMC Biochemistry 2008, 9:24doi:10.1186/1471-2091-9-24


Published:	18 September 2008

Additional files

Additional file 1:

Urea cycle genes in animals. Genomes of animals that belong to different phyla were surveyed for the presence of urea cycle genes. Accession numbers and databases that were searched are listed for each urea cycle gene.

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Additional file 2:

Primers used for site-directed mutagenesis and cloning of NAGS. Table 1 lists primers that were used for site-directed mutagenesis of mouse NAGS and X. campestris NAGS-K. Table 2 lists primers that were used for cloning of the arabidopsis, pufferfish, zebrafish, X. laevis, X. tropicalis and M. maris NAGS, and sizes of amplification products for each primer pair.

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