Figure 7.

YB-1 favors tubulin S assembly. A, Tubulin and tubulin S (5 μM each) were analyzed by native agarose gel (left panel) and 8% SDS-PAGE (right panel). In our conditions, complete digestion of both α and β tubulin were total. B, Turbidimetry plot of tubulin S (5 μM) assembly in the absence (a) or presence of 0.625, 1.25, 2.5 μM YB-1 (b, c, and d, respectively) in buffer M with 20% glycerol.