Additional file 1.

Co-immunoprecipitation of POB1 with Grb2 and 14-3-3 at endogenous level is not EGF dependent. HeLa cells were starved for 4 hours in serum deprived medium and induced by addition of EGF at 100 ng/ml, when indicated. Cells were lysed and treated as described. Protein lysates were immunoprecipitated with anti-POB1 (lane 2,3) and mock antibody in lane 1. The co-immunoprecipitated proteins were separated on SDS-PAGE, transfered onto nitrocellulose membranes and probed with anti-Grb2 or anti-14-3-3 zeta. Input is 5% of the amount loaded in the lanes of co-immunoprecipitations.

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Tomassi et al. BMC Biochemistry 2008 9:21   doi:10.1186/1471-2091-9-21