|
Typical results of DSN purification from crab hepatopancreas. |
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| Stage of purification |
Total protein, mg |
Specific activity, Kunitz/mg |
Activity yield, % |
Fold purification |
|
|
||||
| Kamchatka crab hepatopancreas extract |
1948.0 |
51 |
100 |
1.0 |
| Chromatography on DEAE MacroPrep |
275.2 |
265 |
73 |
5.2 |
| Chromatography on Phenyl Sepharose |
70.0 |
801 |
56 |
15.7 |
| Re-chromatography on Phenyl Sepharose |
10.0 |
4900 |
49 |
96.1 |
| Chromatography on Hydroxyapatite |
8.1 |
5200 |
43 |
102.0 |
| Chromatography on Heparin Sepharose |
1.4 |
29000 |
40 |
568.6 |
| Gel filtration on Sephacryl S-200 |
0.95 |
31500 |
30 |
617.7 |
Anisimova et al. BMC Biochemistry 2008 9:14 doi:10.1186/1471-2091-9-14 |
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