Figure 4.

Determination of steady-state kinetic parameters. Double-reciprocal plot of initial velocity data for holoenzyme MtCS-FMN_ox in the presence of varying concentration of NADH (10, 25, 50, 75, 100, 200, 300 μM). Inset: Michaelis-Menten representation. The data were fitted to equation 1, yielding the following values: K_NADH = 36 ± 4 μM, k_cat = 8.3 (± 0.3) × 10^-3 s^-1 and k_cat/K_NADH = 231 M^-1 s^-1.