|
Kinetic parameters for free AMPD before and after removal of low molecular weight ions and metabolites by centrifugation through Sephadex G50 columns |
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| Before spun columns |
After spun columns |
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|
|
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| S0.5 AMP (mM) |
nH |
S0.5 AMP (mM) |
nH |
|
|
|
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| Control |
2.16 ± 0.11 |
1.76 ± 0.06 |
1.35 ± 0.08a |
2.37 ± 0.17 a |
| Frozen |
2.19 ± 0.15 |
2.76 ± 0.10 a |
1.68 ± 0.21 a |
2.76 ± 0.27 |
|
Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs. a – Significantly different from the corresponding free control value prior to desalting by the Student's t-test, P < 0.05. | ||||
Dieni and Storey BMC Biochemistry 2008 9:12 doi:10.1186/1471-2091-9-12 |
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