|
Kinetic parameters of AMPD in crude extracts of skeletal muscle from control (5°C-acclimated) and 24 h frozen wood frogs. |
||
| S0.5 AMP (mM) |
nH |
|
|
|
||
| Control |
||
| Free AMPD |
2.16 ± 0.11 b |
1.76 ± 0.06 bc |
| Bound AMPD |
0.46 ± 0.03 ac |
1.03 ± 0.07 ac |
| Frozen |
||
| Free AMPD |
2.19 ± 0.15 b |
2.76 ± 0.10 ab |
| Bound AMPD |
1.50 ± 0.19 abc |
1.12 ± 0.09 a |
|
Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs S0.5 is the substrate concentration that produces half-maximal enzyme velocity; nH is the Hill coefficient. Significantly different from a the corresponding value for free AMPD from control frogs, b the corresponding value for bound AMPD from control frogs, or c the corresponding value for free AMPD from frozen frogs using the Student's t test, P < 0.05. | ||
Dieni and Storey BMC Biochemistry 2008 9:12 doi:10.1186/1471-2091-9-12 |
||