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Oligonucleotide primers used for the construction of plasmids. Primers were used in the amplification reactions to generate various DNA fragments or mutations constructs. |
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| Name |
Nucleotide sequence* |
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| F-RoGA |
5'-TTCGAATTCATGCAATTATTCAATTTG-3' |
| R-RoGA |
5'-TTCGAATTCTTAAGCGGCAGGTGCACC-3' |
| R-GAΔ168–604 |
5'-TTGAATTCTCAGTTACCAGTTGGGA-3' |
| R-GAΔ132–604 |
5'-TTGAATTCTCATGTAGATACTTGGT-3' |
| F-PS1 |
5'-CGTAGTTTTTCAAGTTCTTAG-3' |
| R-PS1 |
5'-TCCTTACCTTCCAATAATTC-3' |
| F-GAΔ132–167 |
5'-CAAGTATCTACATCTACAATCTCC-3' |
| R-GAΔ132–167 |
5'-AGGAGATTGTAGATGTAGATACTTG-3' |
| F-GAΔ26–131 |
5'-TTGCTTGTTTCTGCTTCCAAGCCCACTACT-3' |
| R-GAΔ26–131 |
5'-AGTAGTGGGCTTGGAAGCAGAAACAAGCAA-3' |
| F-GAΔ26–145 |
5'-AGAAGTAGAAGGGGCAGCAGAAACAAGCAA-3' |
| R-GAΔ26–145 |
5'-TTGCTTGTTTCTGCTGCCCCTTCTACTTCT-3' |
| F-GAΔ26–160 |
5'-TTGCTTGTTTCTGCTGCTACTTTCCCAACT-3' |
| R-GAΔ26–160 |
5'-AGTTGGGAAAGTAGCAGCAGAAACAAGCAA-3' |
| F-GAΔ26–167 |
5'-TTGCTTGTTTCTGCTTCTACAATCTCCTCA-3' |
| R-GAΔ26–167 |
5'-TGAGGAGATTGTAGAAGCAGAAACAAGCAA-3' |
| F-T165A |
5'-CCAGCTGGTAACTCTACAATCTCCTCA |
| R-T165A |
5'-GGAGATTGTAGAGTTACCAGCTGG |
| F-N167D |
5'-ACTGGTGACTCTACAATCTCCTCATGGATT-3' |
| R-N167D |
5'-GGAGATTGTAGAGTCACCAGTTGGGAAAGT-3' |
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*Underlined letters indicate EcoRI sites. Bold letters indicate mutated nucleotides. | |
Lin et al. BMC Biochemistry 2007 8:9 doi:10.1186/1471-2091-8-9 |
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