Table 1

Table showing IC50 values of different purine and allopurinol-based compounds for XOD inhibition.


(μM ± SEM)


Inhibitor
Hypoxanthine
 Xanthine
 6MP
ALLOPURINOL
0.91 ± 0.01
 2.36 ± 0.03
 1.92 ± 0.03
APT
7.28 ± 0.17
 16.38 ± 0.21
 2.57 ± 0.08
AHMP
5.08 ± 0.04
 17.71 ± 0.29
 0.54 ± 0.01
APP
6.79 ± 0.28
 20.76 ± 0.84
 12.86 ± 0.69

The reaction mixture comprised of 1 ml of 0.2 M, sodium phosphate buffer pH 7.4 containing 10 μM of one of the substrates namely, hypoxanthine, xanthine or 6MP. The concentration range of allopurinol and APP were 0–3 μM and 0–100 μM, respectively for all three substrates. The concentration range of AHMP and APT were 0–100 μM and 0–50 μM, respectively for all three substrates. The reaction was initiated by the addition of 2.8 U/ml of bovine XOD.

Kalra et al. BMC Biochemistry 2007 8:8   doi:10.1186/1471-2091-8-8