Figure 6.

xGINS stimulates DNA synthesis by xPol ε holoenzyme. (A) DNA synthesis reactions (10 μl) contained 200 fmol ³²P-labeled 34-mer primer/65-mer template replication substrate [32], 15 fmol r-xPol ε holoenzyme and 45 fmol (×5), 150 fmol (×10), or 300 fmol (×20) xGINS as indicated. Reactions were incubated at 25°C for the indicated amount of time, terminated by addition of stop solution (5 μl), and analyzed by sequencing gel and autoradiography [32]. The ³²P-labeled 65-mer is the reaction product and the ³²P-labeled 34-mer is the primer.(B) The amount of the reaction products in (A) (65-mer) was quantified by Image analyzer (Fiji).