Figure 1.

p12, p17 and p60 of Xenopus Pol ε interact with the C-terminal region of p260. (A) Schematic representation of wild type (full)- and mutant (ΔC2157, ΔC1054, Δ860N, and ΔCat) forms of xPol ε p260. The conserved catalytic DNA polymerase domain and putative zinc finger domain of Pol ε are indicated. (B) FLAG-tagged p260 was co-expressed with p60, p17 and p12 in insect cells. Cell lysates were prepared and immunoprecipitated with anti-FLAG antibody and the precipitates were subjected to SDS-PAGE followed by immunoblotting with antibodies for each subunit. "Lysates" and "bound" indicate total protein and immunoprecipitated proteins, respectively. (C) Schematic representation of Xenopus Pol ε holoenzyme.