Subcellular localization of hARD2-V5 by immunofluorescence. HeLa cells were transiently transfected with plasmid expressing hARD2-V5, fixed and labelled with anti-V5 and thereafter with Alexa-568-conjugated antibody. Images display hARD2-V5 in red (A and C) and nuclear DAPI staining in blue (B and D). Untransfected cells demonstrating the background staining levels of Alexa-568 can be observed in C next to the transfected cells.
Arnesen et al. BMC Biochemistry 2006 7:13 doi:10.1186/1471-2091-7-13