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Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-α-acetyltransferase

Thomas Arnesen12*, Matthew J Betts3, Frédéric Pendino1, David A Liberles3, Dave Anderson4, Jaime Caro5, Xianguo Kong5, Jan E Varhaug2 and Johan R Lillehaug1

Author Affiliations

1 Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway

2 Department of Surgical Sciences, Haukeland University Hospital, N-5021 Bergen, Norway

3 Computational Biology Unit, BCCS, University of Bergen, N-5020 Bergen, Norway

4 Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA

5 Department of Medicine, Thomas Jefferson University, Philadelphia, PA19107, USA

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BMC Biochemistry 2006, 7:13  doi:10.1186/1471-2091-7-13

Published: 25 April 2006

Additional files

Additional File 1:

Peptide sequence alignment of ARD sequences, produced using T-Coffee.

Format: ALN Size: 16KB Download file

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Additional File 2:

Nucleotide sequence alignment of ARD sequences produced by aligning the nucleotide sequences as per their peptide sequence alignment given in 1.

Format: ALN Size: 45KB Download file

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Additional File 3:

Well aligned region of the alignment in 2 (positions 157 to 642), with genomic kangaroo ARD fragments added. This alignment was run through MrBayes to produce the unrooted tree in 4.

Format: ALN Size: 37KB Download file

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Additional File 4:

Unrooted tree produced by running the alignment in 3 through MrBayes. This tree was rooted by mapping on to the tree of life to produce the tree in figure 3 (see methods). In figure 3 the kangaroo clade has been summarised as one node called 'Kangaroo', and the the two versions of Mouse ARD1 have been replaced by just the Ensembl version. Figure produced using ATV (Zmasek and Eddy, 2001).

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Additional File 5:

Structure of the acetyltransferase domain of hARD2, as modelled by alignment of both hARD2 and PDB entry 1qst to the alignement of Pfam domain PF00583 and then running through SwissModel. The space-filled residue shows the position of the Ala-Pro substitution on going from hARD1 to hARD2, and that this substitution occurs in a loop. Figure produced using MolScript (Kraulis 1991).

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