Figure 2.

Effects of EAAC1 siRNA on EAAC1 expression and aspartate uptake by PC-3 cells. A. The specificity of the EAAC1siRNA is demonstrated by the absence of any effect on EAAT1. B. EAAC1 siRNA knocks down the level of EAAC1 in the membrane fraction of PC-3 cells. Na/K ATPase used as a membrane protein for loading control. siRNA(3) and siRNA(5) are 2 siRNA pool preparations based on difference sequences of EAAC1. C. Specific knock- down of EAAC1 decreases high-affinity uptake of aspartate by PC-3 cells