Table 2

Residues phosphorylated in HSF1 from heat-treated cells.

Peptide

N

Phospho-residue

Dig.

VEEApSPGRPpSSVDTLLpSPTALIDSILR

4

314, 319, 326

Trp.

VKEEPPpSPPQpSPR

2

303, 307

Trp.

GHTDTEGRPPpSPPPTSTPEK

3

363

Trp.

VVHIEQGGLVKPERDDTEFQHPCFLR

1

(97)

Trp.

GHTDTEGRPPpSPPPTSTPEK*

2

363

Trp.

VKEEPPpSPPQpSPR*

2

303, 307

Trp.

QFpSLEHVHGSGPY*

1

230

Trp.

VEEApSPGRPpSSVDTLLpSPTALIDSILR*

3

314, 319, 326

Trp, Chytrp.

VEEASPGRPSSVDTLLpSPTALIDSILR

2

326

Trp, Chytrp.

VEEApSPGRPSSVDTLLpSPTALIDSILR

2

314, 326

Trp, Chytrp.

VKEEPPpSPPQSPR

1

303

Trp, Chytrp.

GHTDTEGRPPpSPPPTSTPEK

2

363

Trp, Chytrp.

KVTpSVSTLKS

1

121

Trp, Chytrp.

PLSSpSPLVR

1

292

Trp, Chytrp.

pSLEHVHGSGPY

1

230

Trp, Chytrp.

DARGHTDTEGRPPpSPPPTSTPEKCLSVACL

2

363

AspN

DERPLSSpSPLVRVK

2

292

AspN

DLFpSPSVTVP

1

419

AspN

DSSLASIQELLpSPQEPPRPPEAENSSP

1

444

AspN

DTLLpSPTALI

1

326

AspN

PASVTALTDARGHTDTEGRPPpSPPPTSTPE

1

363

Glu-C

LLpSPQEPPRPPEAEN

1

444

Glu-C

SEPAPApSVTALTDARGHTDTE

1

344

Glu-C

ApSPGRPSSVDTLLpSPTALID

2

314, 326

Glu-C

LFpSPSVTVPD

1

419

Glu-C

N: Unambiguously identified phosphoresidues are bolded and are preceded by p. Where identification was ambiguous, candidate residues are underlined. Dig.: proteinase used for digestion of HSF1. *Radiolabeled peptide. Trp: trypsin; Chytrp: chymotrypsin. For experimental details relating to analysis of HSF1 phosphopeptides and identification of phosphorylated residues see Methods.

Guettouche et al. BMC Biochemistry 2005 6:4   doi:10.1186/1471-2091-6-4