Table 4

Proteins with highest phosphorylation signal from proteome chip screen for Dbf2-Mob1 substrates

ORF Name

Common Name

Slide Signal (after normalization)

MW (kDa)

# of R-3 Sites


YJR060W

CBF1

118.4224

39

3

YAL051W

OAF1

81.8589

121

3

YBR138C

HDR1

8.9063

61

2

YOL012C

HTZ1

4.0738

14

1

YMR165C

SMP2

3.2182

95

7

YPL150W

3.1545

100

16

YDR226W

ADK1

3.1016

24

0

YMR229C

RRP5

2.6954

193

5

YBR118W

TEF2

2.619

50

0

YJL108C

PRM10

2.5802

41

0

YPR091C

2.5784

87

3

YKL168C

KKQ8

2.5013

84

14

YNL101W

AVT4

2.1486

80

7

YBR285W

1.9355

17

0

YMR239C

RNT1

1.593

54

2

YNR047W

1.4015

100

21

YJL076W

NET1

1.3521

128

11

YNL155W

1.2912

31

1

YNR006W

VPS27

1.1429

71

4

YIL135C

VHS2

1.116

48

11

YMR184W

1.1144

22

3

YDL220C

CDC13

1.0274

105

3

YBR108W

1.0216

93

7

YDL019C

OSH2

0.9962

146

6

YOR362C

PRE10

0.9895

32

0


Of the 67 putative substrates, the 25 putative substrates with the highest relative amount of phosphorylation signal (amount of signal relative to protein expression) as listed were chosen for further study. MW: predicted molecular weight, # or R-3 Sites: number of RXXS motifs

Mah et al. BMC Biochemistry 2005 6:22   doi:10.1186/1471-2091-6-22

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